132 PRACTICAL ORGANIC CHEMISTRY PREPARATION 43. /N(CH3).CH2.CO.OH Creatine. HN:G/ -hH2O Neubauer, Annalen^ 1861, 119, 27. 500 grms. meat. The meat, separated as far as possible from fat, is put through a sausage machine, or finely chopped and digested with -J- litre of water at 50—60°, and well stirred from time to time. It *s filtered through cloth (see Fig. 75, p. 131), and is then digested with a further 250 c.c. of water in the same way, filtered, and the cloth removed from the frame and squeezed out. The filtrate is heated to boiling to coagulate the albumin, and, on cooling, filtered. Basic acetate of lead is carefully added, just sufficient to precipitate the soluble albumin. The liquid, is again filtered through a fluted filter, and the lead removed with hydrogen sulphide, which is passed into the warm licjuicl. The filtrate from the sulphide of lead is concentrated to n thin syrup on the water-bath and then transferred to a vacuum desiccator, where it is left over sulphuric acid. In a short time, especially on the addition of a crystal of creatine, needle-slmpcd crystals begin to separate, and when no further crystallisation is observed, the crystals, which have a brown colour., arc brought on to a porcelain funnel, and washed with a little spirit. They are recrystallised from a little hot water, with the addition of animal charcoal. Yield about I gram. The filtrate from the creatine contains hypoxanthine and sarcolactic acid, but the small quantity of these two constituents render tliem difficult to extract Properties,—Small rhombic prisms ; with difficulty soluble in cold water, readily soluble in hot water. On warming" \vith alkalis, it decomposes into urea and sarcosine, xN(CH3),CH,.COOH HN:C< ' " \STHo KH(CH3).CR,COONa.